BIOL10004 Chapter Notes - Chapter 3: Reduction Potential, Potential Gradient, Joule

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29 Jun 2018
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Enzymes are Biological Catalysts
Chemical reactions accelerated by biological catalysts
Molecules present surfaces for reactants to bind in specific way
Lowers activation energy of reactions
Biological catalysts are proteins
oENZYMES
Can be common
Can be carrying out particular functions
Reactants = Substrates
Highly specific in way they function
Acts selectively on only one substrate
Enzymes reduce activation energy to allow reactions to occur at
physiological temperatures
Enzymes have high catalytic power
oCarbon dioxide + water forms Carbonic acid
oCatalysed by carbonic anhydrase (ase = enzyme)
CO2 + H2O ↔H2CO3
Absence of Anhydrase = 200 molecules / hour
Anhydrase = 600 000 molecules / second
Enzyme catalysed reaction = 10 million times faster than non-catalysed
reaction
oFirst step in enzyme-catalysed reaction
Binding of substrate or substrates to surface of enzyme protein
Forms a transient enzyme-substrate complex (ES)
In the ES, chemical reaction occurs
E + S ↔ ES
Enzyme + substrate ↔ Enzyme-substrate complex
ES --> E + P
Product
Oxidation and reduction reactions
Oxidation is the addition of oxy gen, removing electrons or removing hydrogen
Reduction is the removal of oxygen, adding electrons or adding hydrogen
oReductions add energy to molecules
oOxidations release energy from molecules
Interconversion of Ferrous (Fe2+) and ferric (Fe3+) ions
Involves loss or gain of single electron
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Document Summary

Molecules present surfaces for reactants to bind in specific way. Enzymes reduce activation energy to allow reactions to occur at. Enzymes have high catalytic power physiological temperatures o o. Absence of anhydrase = 200 molecules / hour. Enzyme catalysed reaction = 10 million times faster than non-catalysed reaction o. Binding of substrate or substrates to surface of enzyme protein. Oxidation is the addition of oxy gen, removing electrons or removing hydrogen. Reduction is the removal of oxygen, adding electrons or adding hydrogen o. Reductions add energy to molecules: oxidations release energy from molecules. Interconversion of ferrous (fe2+) and ferric (fe3+) ions. Involves loss or gain of single electron: donor has tendency to give up electrons, acceptors have tendency to take up electrons. Tendency to accept or donate is due to electrical potential of the redox couple. Oxidation - reduction potential is expressed in volts or millivolts.

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