BIOL10004 Chapter Notes - Chapter 6: Citric Acid, Glyoxylic Acid, Succinyl-Coa

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29 Jun 2018
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Harvesting Chemical Energy - Respiration
Harvesting Chemical Energy
Energy metabolism common to both autotrophs and heterotrophs
Conversion of chemical energy of fuel to useable energy as ATP (adenosine Triphosphate)
oBoth organisms use ATP to drive cellular functions
oPhotosynthetic plants have capacity to release energy from high-energy molecules
ATP made through metabolism of energy-rich molecules
oCarbohydrates
oFats
Energy released along metabolic pathways that are initially different for carbohydrates and
lipids
oCarbohydrates converted to glucose via Glycolysis
oLipids converted by β-Oxidation
Products of both reactions are substrates for Cellular Respiration
Cellular respiration = removal of electrons from:
oC-C bonds
oC-H bonds
Electrons extracted are accepted by co-enzyme NAD+ and FAD (Flavin Adenine Dinucleotide)
oThen passed down electron transport chains
oReaches final electron acceptor
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oDrives proton pumps that coupled to synthesis of ATP
1. Glucose metabolised by glycolysis in cytosol of cell (net = 2 ATP)
2. 3-carbon pyruvate enters different pathway
3. Absence of oxygen, pyruvate converted to lactate or ethanol
4. Presence of oxygen, energy in pyruvate converted in mitochondrion to form 34-36
ATP
5. Energy extraction pathway is citric acid cycle and electron transport chain
6. Electrons transferred to coenzyme NAD+ and FAD
7. Then used to drive proton pumps on inner membrane of mitochondria (ATP
synthesis)
Lipids first metabolised by β-oxidation, product Acetyl CoA being oxidised by same pathway
as pyruvate from glycolysis
Glycolysis : the initial processing of glucose
Polysaccharides (starch + glycogen)
oBroken down by catalysed hydrolysis into glucose
oEnters energy releasing pathways
In all cells (prokaryotic + eukaryotic) glucose processed in cytosol by glycolysis
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Glycolysis involves 2 stages
oOne that consumes energy
oOne that releases energy
More energy is released than consumed and glycolysis is net producer of energy
Stage one
oTwo ATP molecules used to phosphorylate and change glucose
oPreparation for splitting into 2 3-carbon molecules (glyceraldehyde-3-phosphate)
Stage two
oOxidation of glyceraldehyde-3-phosphate to pyruvate
oATP synthesis; four ATP molecules produced
oGiving net energy profit of 2 ATP molecules
o4 electrons and 2 hydrogen atoms transferred to two molecules of NAD+
oProduces two molecules of NADH
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Document Summary

Energy metabolism common to both autotrophs and heterotrophs. Conversion of chemical energy of fuel to useable energy as atp (adenosine triphosphate) o o. Both organisms use atp to drive cellular functions. Photosynthetic plants have capacity to release energy from high-energy molecules. Atp made through metabolism of energy-rich molecules o o. Energy released along metabolic pathways that are initially different for carbohydrates and lipids o o. Products of both reactions are substrates for cellular respiration. Cellular respiration = removal of electrons from: o o. Electrons extracted are accepted by co-enzyme nad+ and fad (flavin adenine dinucleotide) o o. Reaches final electron acceptor: drives proton pumps that coupled to synthesis of atp. Glucose metabolised by glycolysis in cytosol of cell (net = 2 atp) Absence of oxygen, pyruvate converted to lactate or ethanol. Presence of oxygen, energy in pyruvate converted in mitochondrion to form 34-36. Lipids first metabolised by -oxidation, product acetyl coa being oxidised by same pathway as pyruvate from glycolysis.

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