BIOL10004 Chapter Notes - Chapter 6: Bacteriochlorophyll, Manganese, Ribulose

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jadebutterfly872

29 Jun 2018

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University of Melbourne

Department

Biology

Course

BIOL10004

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All

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Harvesting Light Energy: Photosynthesis

Photosynthesis is the process which solar energy harvested and stored in chemical bonds of

ATP and carbohydrates, made from CO2 and H2O

Chlorophyll is the light absorbing pigment in photosynthetic organisms

Eukaryotes

oChlorophyll associated with thylakoid membranes of chloroplast

Chloroplasts have wide variety of sizes and shapes,

Overall Process of photosynthesis involves chemical reactions

In Eukaryotes, 3 essential chemical processes in photosynthesis

1. Absorption of energy from sunlight by pigments-light depending reactions:

Harvesting of light energy through Photosystem 1 and Photosystem 2

Consists of protein molecules in pigments held in special arrangements

Photosystems embedded in thylakoid membranes of chloroplasts

Light energy absorbed by pigment molecules

Transferred to special part of photosystem , the reaction centre

Electrons here energise and removed to be used in chain reactions

Energy stored in molecules of ATP and NADPH

2. Reactivation of reaction centres:

Reaction centres reactivate

Replaces electrons removed follow light energy absorption

Water acts as source of electrons

Oxygen produce as by-product

3. Carbon fixation to produce carbohydrates in 'dark reactions'

Reactions are light independent

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Uses energy from ATP and NADPH

Synthesise sucrose and starch

Reactions function in darkness and in light

Depends on ATP and NADPH has been generated in light reactions

Light energy

Light is form of EMR

oHas wave and particle properties

oWave nature described in terms of wavelength/frequency

oWhen interacting with matter, light = photons (discrete packages of energy)

Photos have different amounts of energy

Inversely proportional to wavelength of photo

Blue photos wavelength = 400nm, more energy than red photos = 700nm

Sunlight is mixture of photons with range of energy levels

oSome are perceived by eyes in visible range

oObject that is red, absorbs all other spectrums and reflects red

Light used in photosynthesis falls within visible spectrum

Below visible spectrum, photons have very short wavelengths

Above visible spectrum, photons have very long wavelengths

Pigments: molecules that absorb light

Pigments are coloured molecules

oSeen as coloured because photos with particular wavelengths are absorbed, others

reflected

oChlorophyll is green because absorbs red and blue photons

Reflects green photons

Pigment is characterised by pattern of absorption of photos - Absorption Spectrum

oAbsorption spectrum of chlorophyll similar to wavelengths of light that activate

photosynthesis

Action Spectrum

Chlorophyll is main pigment involved in photosynthesis

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Chlorophyll absorbs light by process of excitation

oCentral metal atom (magnesium)

oSurrounded by alternating single/double bonds

oForms a Porphyrin Ring

Photons absorbed by pigment

Electrons in magnesium atom excited

Energy funnelled off through the bonds of the porphyrin ring

oChlorophyll a is principal photosynthetic pigment

Found in cyanobacteria, algae and plants

oPhotosynthetic bacteria

Pigment called Bacteriochlorophyll is found

oGreen algae and plants

Also contain chlorophyll b

Slightly different absorption spectrum from chlorophyll a

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Related Questions

Photosynthesis

Light Reactions:

Photosynthesis is the process of reducing/oxidizing (circle one) _____________________ to produce ___________________. This overall reduction-oxidation reaction requires _________________ to begin the flow of __________________. Photosynthetic ____________________ absorb light energy and are located in ___________________ found embedded within the membranes of __________________. The most prevalent one include the ___________________ which reflect ________________ light and absorb the other wavelengths of light. Accessory pigments include ___________________. When light energy is absorbed in photosynthesis, electrons of pigment molecules become _________________ and move into higher potential energy _______________. When the electrons return to their previous position, _____________ is passed to the ______________________ pigment molecule. Electrons within this pigment molecule move to a high potential energy state and are âgrabbedâ by _________________________ chains. Electrons from photosystem II are passed to ______________________. Electrons from photosystem I are passed to _________________. As electrons move in the electron transport chain associated with photosystem I/II (circle one), the energy released is used to power ___________ pumps and __________ is produced via a process known as ________________________. Photosystem I/II (circle one) electrons must be replaced to continue the reaction so ____________ is split for its electrons. The products _______________ & _______________ then proceed to the ______________________ cycle.

Calvin Cycle:

The Calvin Cycle can be broken into 3 main series of reactions. In the first group, known as the __________________ step, ______________________ combines with 3 molecules of ____________________ to form 6 molecules of _____________________. This reaction is catalyzed by ___________________. ________________ can act as a competitive inhibitor of this enzyme, shutting down this step in a process known as ______________________________. In the second series of reactions known as the __________________ steps, the molecules produced in the previous step get oxidized/reduced (circle one). The source of electrons is _________from the light reactions. This step is endergonic/exergonic (circle one) and thus requires _______ from the light reactions. Six molecules of _____________________ are produced, ______ (#) of which will continue in biosynthetic pathways to produce __________________. The remaining molecules will then enter the ______________________ steps which require _____________ from the light reaction. In this step, 3 molecules of _______________ are produced so that this cyclic reaction can continue.

The complete set of reactions carried out by an organism is called:

a. anabolism

b. catabolism

c. metabolism

Value: 2

The breakdown of starch is broken down to glucose is an example of:

a. anabolism

b. catabolism

c. metabolism

Value: 3

For the following statement, determine which description best describes the process: DNA is synthesized from free nucleotides.

a. anabolism

b. catabolism

c. metabolism

Value: 4

Fill in the blank: Catabolic reactions result in the synthesis of molecules of .............via cellular respiration. This is then used to power anabolic reactions.

Value: 5

The energy in organic molecules is released in a series of steps because:

a. it is not possible to release it in a single step.

b. more total energy is released in multiple steps than would be released in a single step

c. less total energy is released in multiple steps than would be released in a single step.

d. more energy can be harvested for cellular use in multiple steps than from a single step.

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photosynthesis

1-The light reaction of photosynthesis does not include

a. chemiosmosis

b. oxygen liberation

c. glucose synthesis

d. electron transport

Value: 2

Photosynthesis takes place in the membranes of small sacs called

a. thylakoids

b. photosystems

c. stroma

d. photons

Value: 3

During what stage of photosynthesis is O2 produced?

a. the light-dependent reactions involving photosystems I and II

b. carbon fixation

c. the Krebs cycle

d. O2 is not produced during photosynthesis

Value: 4

The pigment molecules responsible for photosynthesis are located in the

a. mitochondria

b. cytoplasm of the cell

c. stroma of the chloroplast

d. thylakoid membrane of the chloroplast

Value: 5

During what stage of photosynthesis are ATP and NADPH converted to ADP + Pi and NADP+?

a. the light-dependent reactions

b. the light-independent reactions

c. both of the above

d. none of the above

Value: 6

What energy-rich organic compound is produced as a result of the Calvin cycle?

a. ATP

b. NADPH

c. glucose

d. H2O

Value: 7

During photosynthesis, photons raise electrons to higher energy levels. These excited electrons belong to what compound?

a. water

b. ATP

c. chlorophyll

d. electron transport chain

Value: 8

Which of the following statements accurately describes the relationship between photosynthesis and cellular respiration?

a. photosynthesis occurs only in autotrophs; cellular respiration occurs only in heterotrophs

b. photosynthesis uses solar energy to convert inorganics to energy-rich organics; respiration breaks down energy-rich organics to synthesize ATP

c. photosynthesis involves the oxidation of glucose; respiration involves the reduction of CO2

d. the primary function of photosynthesis is to use solar energy to synthesize ATP; the primary function of cellular respiration is to break down ATP and release energy

Review question its 1-12 sorry for the xtra questions in the bottom

1:2D chromatography is so named because ____

A: there are two steps: labeling with a radioactive marker and separation via chromatography

B: two different solutions are used in the paper chromatography

C: the molecules are separated into a linear arrangement, meaning two dimension

D: two different types of radioactive markers must be used

E: the process separates the molecules in two direction, first along the X and then along the Y axis

2: :Which molecule(s) regulate(s) photosynthesis by sensing the metabolic status of the chloroplast and regulating proton flow out of the thylakoid lumen??

A: ATP synthase

B: antenna complex

C: Chrolophyll B

D: carotenoids

E: chlorphyll A

3: When does A CAM plant take in atmospheric CO2?

A: daytime only

B: Summer day

C: winter day

D: nighttime only

E: anytime the calvin cycle is happening

4: Where can you find ATP synthase in a plant cell ?
A; in the thylakoid membrane only

B: in the mitochondial inner membrane

C: both thylakid and inner mitochondria membrane

D: only in the nucleus

E: embedded in the plasma membrane

5: chloroplast kept in the daek can continue to make sugar is provided with

A: NADPH, CO2, and ADP

B: CO2, and ATP

C: NADPH, ATP, and CO2

D: NADPH and ATP

E: water and ATP

7: C3 plants are more susceptible to photorespiration than either C4 or CAM plants. Why do most plants lack the C4 pathway??

A: Switching between C3, C4, and CAM photosynthesis is very difficult for most plants

B: ?Rubisco is more efficient in C3 plants when compared to rubisco of C4 and CAM plants

C: Most plant species do not have vacuoles in their cells to store the malate generated from CO2 taken in during the night.

D; : Photorespiration is not a serious problem for most plants.?

E: There is an energetic cost to C4 and CAM pathways that only makes them advantageous in very hot and/or arid environments.?

8: In Engelmann's classic experiment, why were the oxygen-requiring bacteria clustered around the regions of Spirogyra algae that were bathed in red, blue, and violet light?

A; The bacteria were immobile and that is where Engelmann happened to place them

B; The most energy-rich wavelengths of light are found at the edges of visible and nonvisible light (ultra

violet and infrared)

C; Photosynthesis is most active in those wavelengths of light and thus more oxygen is consumed by the algae in those regions.

D: Photosynthesis is most active in those wavelengths of light and thus more oxygen is produced by the algae in those regions.?

E: : Chlorophyll is green and thus reflects green light.

9: In any experiment, changes to the dependent variable based on the manipulations of the independent variable are measured. When determining the action spectrum of a given plant, what is the independent variable and what is the dependent variable?

A: independent = rate of O2 release in photosynthesis; dependent = wavelength?

B: ?independent = absorption of light; dependent = wavelength

C: independent = wavelength; dependent = rate of O2 release in photosynthesis?

D: independent = wavelength; dependent = absorption of light?

E: independent = rate of O2 release in photosynthesis; dependent = absorption of light

10: Which plant structure is responsible for gas exchange??

A: stromal lamellae

B: grana

C: granastomata?

D: stroma

11: n order to elucidate the Calvin cycle, Calvin and his colleagues used two-dimensional paper chromatography to analyze the presence of different molecules under different conditions. What did they conclude from the accumulation of RuBP when CO2 levels were low??

A: CO2 serves as a non-competitive inhibitor of rubisco.

B: RuBP is the last substrate to react in the Calvin cycle.

C: RuBP only reacts with CO2 when concentrations of ATP are low

D: RuBP is the first substrate to react with CO2

E: ?RuBP only reacts with CO2 in the sunlight.

12: Why isn't oxygen produced by organisms that perform anoxygenic photosynthesis?

A; their membranes lack ATP synthase?

B; They use a different enzyme for the initial reactions of the calvin cycle

C: they use electrons donors other than water

D: their photosystem do not utilize the calvin cycle

E: their chorophyll contains an iron rather than magnesium

A: ?there are two steps: labeling with a radioactive marker and separation via chromatography

B: two different solutions are used in the paper chromatography process?

C: the molecules are separated into a linear arrangement, meaning two dimensions?

D: two different types of radioactive markers must be used?

E: the process separates the molecules in two directions, first along the x axis and then along the y axis?

2:Which molecule(s) regulate(s) photosynthesis by sensing the metabolic status of the chloroplast and regulating proton flow out of the thylakoid lumen??

A: ?ATP synthase

B: the antenna complex?

C: chlorophyll b?

D: carotenoids?

E: chlorophyll a

3: When does a CAM plant take in atmospheric CO2??

A: ?daytime only

B: summer only?

C: ?winter only

D: nighttime only?

?e: anytime the Calvin cycle is occurring

4: Where can you find ATP synthase in a plant cell??

A: ?in the thylakoid membrane only

B: in the mitochondrial inner membrane only?

C: both the thylakoid and inner mitochondrial membranes?

D: only in the nucleus?

E: ?embedded in the plasma membrane

5: Chloroplasts kept in the dark can continue to make sugar if provided with ____.

A: NADPH, CO2, and ADP?

B: CO2 and ATP?

?c: NADPH, ATP, and CO2

D: NADPH and ATP?

E: water and ATP

6: Where does the Calvin cycle occur?

A: thylakoid lumen?

B: cytosol?

C: stroma?

D: stromal lamellae

?e: thylakoid membrane

7: C3 plants are more susceptible to photorespiration than either C4 or CAM plants. Why do most plants lack the C4 pathway??

A: Switching between C3, C4, and CAM photosynthesis is very difficult for most plants.?

B: ?Rubisco is more efficient in C3 plants when compared to rubisco of C4 and CAM plants.

C: Most plant species do not have vacuoles in their cells to store the malate generated from CO2 taken in during the night.?

D: Photorespiration is not a serious problem for most plants.?

E: There is an energetic cost to C4 and CAM pathways that only makes them advantageous in very hot and/or arid environments.?

8: In Engelmann's classic experiment, why were the oxygen-requiring bacteria clustered around the regions of Spirogyra algae that were bathed in red, blue, and violet light??

A:The bacteria were immobile and that is where Engelmann happened to place them.?

B: The most energy-rich wavelengths of light are found at the edges of visible and nonvisible light (ultra violet and infrared).?

?c: Photosynthesis is most active in those wavelengths of light and thus more oxygen is consumed by the algae in those regions.

D; Photosynthesis is most active in those wavelengths of light and thus more oxygen is produced by the algae in those regions.?

?e: Chlorophyll is green and thus reflects green light.

9; In any experiment, changes to the dependent variable based on the manipulations of the independent variable are measured. When determining the action spectrum of a given plant, what is the independent variable and what is the dependent variable??

A: independent = rate of O2 release in photosynthesis; dependent = wavelength?

B: ?independent = absorption of light; dependent = wavelength

C: independent = wavelength; dependent = rate of O2 release in photosynthesis?

D: independent = wavelength; dependent = absorption of light?

E: independent = rate of O2 release in photosynthesis; dependent = absorption of light?

10: Which plant structure is responsible for gas exchange??

A: stromal lamellae?

B: grana?

C: stomata?

?D: stroma

11: In order to elucidate the Calvin cycle, Calvin and his colleagues used two-dimensional paper chromatography to analyze the presence of different molecules under different conditions. What did they conclude from the accumulation of RuBP when CO2 levels were low??

A:	?CO2 serves as a non-competitive inhibitor of rubisco.

?B: RuBP is the last substrate to react in the Calvin cycle.

C: RuBP only reacts with CO2 when concentrations of ATP are low.?

D:	RuBP is the first substrate to react with CO2.?

e:	?RuBP only reacts with CO2 in the sunlight.

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BIOL10004 Chapter Notes - Chapter 6: Bacteriochlorophyll, Manganese, Ribulose

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