One question, double check section as well.
Several genes code for enzymes that are responsible forhistidine biosynthesis in Escherichia coli. Part of theamino acid sequence for one of these enzymes is:
AUG CCU UGG GCC CAA AAA UGC
A series of mutants that have lost activity for this enzyme arerecovered. The mutant products are analyzed and found to have thefollowing amino acid sequences:
Mutant 1: Met-Pro-Trp-Pro-Glu-Lys-Cys
Mutant 2: Met-Pro
Mutant 3: Met-Pro-Cys-Gly-Pro-Lys-Met
Mutant 4: Met-Pro-Trp-Pro-Lys-Asp
Choose the correct type of mutation that occurred in the DNA toproduce each mutant type.
Nonsense :
Point :
Addition :
Deletion :
________________
Double check section:
A small section of Saccharomyces cerevisiae gene has the aminoacid sequence valine, histidine, cysteine, and lysine.
A mutation in the above section of the amino acid sequence resultedin the substitution of amino acid lysine with amino acidasparagine.
The mutation in the antisense strand DNA of Saccharomycescerevisiae gene described above involves
Select one:
a. the substitution of second adenine base from AAG
b. the substitution of cytosine base from TTC
c. the substitution of guanine base from AAG
d. the substitution of second thymine base from TTC
*** I picked c, is that correct?
A small section of a gene for a protein has the followingnucleotide sequence:
TAT AGG GAC CTA TGT
Which of the following mutations would cause a missense mutation inthe sequence shown above?
Select one:
a. Replacement of second guanine base with cytosine base
b. Replacement of first cytosine base with guanine base
c. Replacement of final thymine base with guanine base
d. Replacement of first thymine base with adenine base
** Picked A
Which of the following was not one of the first uses of modernbiotechnology?
Select one:
a. The insertion of a bacterial insulin gene into humancells.
b. The cloning of a mammal.
c. The insertion of an antifreeze gene into a fish.
d. The insertion of a human insulin gene into bacteria.
** I picked A
What are the sticky ends of the restriction fragments?
Select one:
a. The surfaces of sticky ends contain matching base pairs,allowing fragments to splice.
b. The surfaces of sticky ends have glue like substance thatallow fragments to splice.
c. The surfaces of sticky ends contain the exact samenucleotides, allowing fragments to bond.
d. The surfaces of sticky ends have velcro like structure,allowing fragments to bond.
** a
