BIOL 367 Chapter Notes - Chapter 14: Guanine, Alternative Splicing, Koin
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Rna pol cannot distinguish the coding region of the gene from the noncoding regions, so it transcribes everything. Thus, the cell must remove the noncoding rna from the original transcript, a process called splicing. Eukaryotes also tack a special cap and polya tail. In r-looping experiments, rna is hybridized to its dna template. The dna template strands are separated to allow a double-stranded hybrid to form between one of these strands and the rna product. Such a hybrid is a bit more stable than a double stranded dna. After the hybrid forms, it is examined by electron microscopy. If the hexon gene had no introns, a smooth, linear hybrid would occur where the mrna lined up with its dna template. The fork at the lower right is due to the (cid:1007)" e(cid:374)d of the (cid:373)rna, whi(cid:272)h (cid:272)a(cid:374)(cid:374)ot h(cid:455)(cid:271)ridize (cid:271)e(cid:272)ause the (cid:1007); e(cid:374)d of the ge(cid:374)e is not included.
