BIOCHEM 2B03 Chapter Notes - Chapter 29: Rna Polymerase Iii, Ribosomal Rna, Thermophile
6 Sep 2013
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Chapter 29: transcription and the regulation of gene expression. Gtp, ctp, utp) in an order specified by base pairing with a dna template: n ntp (nmp)n + n ppi, rna pol moves along dna strand in the 3" 5" direction, joining the. Prokaryotic rna polymerases use their sigma subunits to identify sites where transcription begins. Nucleophilic attack of 3"-oh of preceding nucleotide on the -phosphate of the incoming nucleoside triphosphate. Pyrophosphate is released and hydrolyzed by inorganic pyrophosphatase. Chain initiation: pppn1 + pppn2 pppn1pn2 + ppi. Chain elongation: pppn1pn2 + pppn3 pppn1pn2pn3 + ppi. Pppn = atp, ctp, gtp, and utp as determined by the dna template. E. coli rnap found in at least two forms: holoenzyme and core enzyme. 2 " holoenzyme promoter recognition and initiation. 2 " core enzyme rna synthesis but cant initiate (cannot recognize promoters) And " form a catalytic claw that dna passes through. Binds ntps and interacts with .
