BIOLOGY 1A03 Chapter Notes - Chapter 19: Restriction Enzyme, Reverse Transcriptase, Recombinant Dna

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Rosa Da Silva

Document Summary

Treatment: prion: infectious proteins that form sponges. Banning the use of cadavers for hgh was banned which led to the rise of genetic engineering. Genetically engineer a bacteria and a shuttle mechanism and grow in yeast. Rna-dependent dna polymerase: requires a rna template to construct a dna sequence. Can use cdna to construct a protein. Antibiotics do not fully destruct bacteria rather it reduces the population first. Plasmids can be used to identify bacteria from another species. Selectable marker: ensures that the bacteria being studied does not need to share nutrients with other bacteria: since the other bacteria will not survive the use of antibiotics. Sticky ends: cuts the dna in a way that creates an overhang. Ensure that the ends are compatible for fusing. Dna ligase will seal the ends together. The produced molecule is now self-replicating and immune. Also need a stop codon to signal when to terminate duplication.