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Biological Sciences
BISC 202
Timothy Swartz

8RNA Transcription and Processing WORKING WITH THE FIGURES1 In Figure 83 why are the arrows for genes 1 and 2 pointing in opposite directionsAnswer The arrows for genes 1 and 2 indicate the direction of transcription which is always 5 to 3The two genes are transcribed from opposite DNA strands which are antiparallel so the genes must be transcribed in opposite directions to maintain the 5 to 3 direction of transcription 2 In Figure 85 draw the one gene at much higher resolution with the following components DNA RNA polymerases RNAsAnswer At the higher resolution the feathery structures become RNA transcripts with the longer transcripts occurring nearer the termination of the geneThe RNA in this drawing has been straightened out to illustrate the progressively longer transcripts 3 In Figure 86 describe where the gene promoter is locatedChapter Eight 271 Answer The promoter is located to the left upstream of the 3 end of the template strand From this sequence it cannot be determined how far the promoter would be from the 5 end of the mRNA 4 In Figure 89b write a sequence that could form the hairpin loop structure nverted complementary regions separatedAnswer Any sequence that contains iby a noncomplementary one would form a hairpinOne sequence would beACGCAAGCUUACCGAUUAUUGUAAGCUUGAAGThe two boldfaced sequences would pair and form a hairpinThe intervening nonbold sequence would be the loop 5 How do you know that the events in Figure 813 are occurring in the nucleusAnswer The figure shows a doublestranded DNA molecule from which RNA is being transcribed This process only occurs in the nucleus 6 In Figure 815 what do you think would be the effect of a G to A mutation in the first G residue of the intronAnswer A mutation of G to A would alter the U1 SNP binding site and prevent formation of the spliceosomeThis would prevent splicing of the intron 7 In Figure 823 show how the doublestranded RNA is able to silence the transgene What would have to happen for the transgene to also silence the flanking cellular gene in yellowAnswer a The doublestranded RNA formed from the sense and antisense transgene transcripts would be processed by Dicer then bind to RISCRISC would separate the dsRNA to produce an antisense RNARISC complex The RISCRNA complex would bind to the transgene mRNA and deactivate it
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