MBG 2040 Chapter Notes - Chapter 10: Endonuclease, Dnaa, Telomere
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2 Dec 2016
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Preformed an experiment to prove the method of dna replication. The results were that dna replication is semiconservative and the experiment was as follows: e. coli was grown in n15 medium, the n15 dna was then grown on an. N14 medium: n15 is heavier than the light n14 so using an equilibrium gradient centrifugation they were able to separate dna based on density. The ratio was determined by measuring the band width produced by the centrifuge. It is highly processive and speeds up the addition of nucleotides. Dna synthesis occurs in 5" 3" direction. Starts at the origin, oric, which contains tandem 13-bp sequences and four 9-bp initiation protein sites. Replication does not occur at a definitive phase. Dnaa protein: initiator protein binds to 9-bp sequence in oric and forms complex. Helicases: catalyzes the unwinding of the parental double helix (5" 3") Ligase: closes the nicks in dna in the lagging strand.
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