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Chp.5 summary Psyc 2410

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PSYC 2410
Elena Choleris

PSYC 2410-01 9/26/2013 6:55:00 AM 2-DG (2-deoxyglucose)- modified form of glucose picked up by active cells  The more active the cell, the more 2-DG it will pick up  Discussed in relation to PET scans Autoradiography- tell you which brains areas have become selectively active due to the behaviours of interest  Lidocaine diffusion- used to temporarily silence a specific region of the brain o Wanted to learn the size of the brain area affected by this diffusion so he added radioactivity to the Lidocaine which demonstrated the degree of the spread of the drug Cerebral Dialysis- insert inner cannula and get your subject to perform a specific behaviour. While the subject is doing this, collect extracellular fluids from their brain. Then observe the different neurochemicals that were released during the specific task. Allows you to chemically measure what is going on in the brain Locating Neurotransmitters and Receptors:  Uses dyes/radioactive labels o Autoradiography o Green fluorescent protein- not easily visible under normal light, however quite clear under fluorescent  Discovery won the Nobel Peace Prize in Chemistry o Brainbow mice- different neurons express different coloured proteins; demonstrates the interconnection of neurons  Immunocytochemistry o Antibodies- specific proteins produced by the body in response to foreign proteins being introduced into the body o Take antibodies, attach a tag, attach to a tissue, and then follow the coloured path to the protein  Demonstrates the location of the foreign protein  In Situ Hybridization- identifies RNAs o Identifies the activity of the gene (one step closer to identifying the transcription of the gene)  RNA binds to the specified compliment carrying the radioactive signal which allows the observer to locate the desired RNA Neuropsychological Testing Approaches: Single-test  Aim was assessing the cause and consequences of brain damage  Approach no longer followed because it does not assess the presence of multiple damages Standardized-test battery  Test neurological and normal patients and compare the differences  Most common approach  Common Tests: o Weschler Adult Intelligence Scale (WAIS) IQ
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