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Chapter 17

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BIOL 112
Karen Smith

Chapter 17 Notes Control of Gene Regulation - Bacteria 17.1 Gene Regulation and Information Flow DNAmRNAProteinActivated Protein 1. Transcriptional Control: DNA—X-> mRNA (Slowest, Most effective-no use of energy) 2. Translational Control: mRNA—X-> protein 3. Post-translational Control: protein—X->activated protein (Fastest, lots of energy needed) - Constitutively: all the time, referring to genes that are transcribed all the time 17.2 Identifying the Genes Involved in Lactose Metabolism - LacI: regulatory protein to allow a set of genes to be transcribed - 3 genes (lacZ,lazY,lacA) code for three proteins (LacZ,LacY,LacA) lacZ codes for LacZ : a beta-glactosidase- this enzyme cleaves lactose into glucose and glactose lacY codes for LacY: a permease that transports lactose across the cell membrane lacA codes for LacA: transacetylase (LacA is not involed in lactose untilization, function unknown) 17.3 Mechanisms of Negative Control: Discovery of the Repressor - negative control: regulatory protein binds to DNA (upstream the promoter) and shuts down transcription, it must be taken away for transcription to occur - lacI gene = repressor as it exerts negative control over lacZ and lacY to inhibit transcription The lac Operon - Operon: a region of prokaryotic DNA that codes for serious of functionally related genes and is transcri
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