BIOL 201 Chapter Notes - Chapter 2-3: Affinity Chromatography, Peptide, Dielectric

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9 Apr 2018
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Parameters affecting the nci dispersion any polarizability; size; molecular weight dipole-dipole species with permanent strength and orientation of dipoles (c=o, etc) dipole hydrogen bond. H bonded to n, o, or f and a orientation lone pair on n, o, or f ionic interaction ions; charged species dielectric size; type of charge. Peptide bonds are amide linkages polymers of amino acids formation is a condensation reaction form from nucleophilic attack by an electron pair on an (cid:536)-amino nitrogen atom on an (cid:536)-carboxyl carbon atom of another amino acid ( oh leaving group) Tertiary structure: overall shape of a single polypeptide unit. Quaternary structure: overall macromolecule structure containing more than one polypeptide chain. Secondary structure (cid:536) helices (cid:537) sheets (cid:537) turns. 1 st residue hydrogen-bonded to 5 th residue (residue n is h-bonded to residue n +4) all nh groups point in the same direction successive r groups point in opposite direction.

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