Chem 313 Problem Set #5, 2010 ANSWERS
1. IPTG is a common chemical used for the induction of the lac-operon in many
recombinant gene technologies. How is IPTG prepared from galactose?
2. Outline the steps you would take to prepare each of the four following
disaccharides (starting from any appropriate unprotected carbohydrate precursor): 3. X-Gal is a commonly used reagent in bacterial cloning technologies. It produces a
convenient blue product (after oxidation within the cell, shown on the right) to easily
identify a link between phenotyp