CHEM 313 Chapter Notes -Oligonucleotide Synthesis, Dehalogenase, Acetic Anhydride
Document Summary
Chem 313 problem set #6 2010 answers: the synthesis of polynucleotides has been studied and refined for years. Some of the original work in this field was carried out at ubc, by h. g. His lab"s methods of forming the phosphodiester backbone of polynucleotides involved the use of dicyclohexylcarbodiimide (dcc) with 5" phosphorylated precursors. A potential byproduct of this reaction is the formation of 5"-3" cyclic phosphates. What is the mechanism of this reaction: the rate of acidic hydrolysis of trityl ethers is very dependent on the substituents on the phenyl rings. Predict (using >,< or =) the relative rate of each of the following reactions. Ome is an edg and stabilizes the carbocation through resonance. Reaction of either of these derivatives using the conditions to form c gives the same, expected product. B shows two products due to the tetrahedral nature of phosphorous.
