BIO206H5 Chapter Notes - Chapter 10: Restriction Enzyme, Recombinant Dna, Dna Replication
Document Summary
These technical breakthroughs dubbed recombinant dna technology or genetic engineering have has a dramatic impact on all aspects of cell biology: have advanced our understanding of the organization and evolutionary history of complex eukaryotic genomes. Led to the discovery of whole new classes of genes, rnas, and proteins o: continue to generate new ways of determining the functions of genes and proteins in living organisms. Recombinant dna technology has also had a profound influence on our understanding and treatment of disease: used to produce an increasing number of pharmaceuticals. Isolating and manipulating individual genes is not a trivial matter: unlike a protein, a gene does not exist as a discrete entity in cells; it is a small part of a much larger dna molecules. Restriction nucleases cut dna molecules at specific sites. A search for the mechanism responsible for degrading foreign dna in bacteria revealed a novel class of bacterial nucleases that cleave dna at specific nucleotide sequences.
