Textbook Notes (367,969)
Canada (161,538)
Biology (653)
BIO152H5 (140)
Chapter 15

Chapter 15 How Genes Work.docx

2 Pages
Unlock Document

Fiona Rawle

Chapter 15 How Genes Work 15.1 What Do Genes Do? Knockout mutants/null mutants/loss-of-function mutants: Alleles that do not function at all. One-gene, one-enzyme hypothesis: Genes contain the information needed to make proteins, many of which function as enzymes. Genetic screen: Technique for picking certain types of mutants out of many thousands of randomly generated mutants. *Figure 15.2 Experimental support for the one-gene, one-enzyme hypothesis. 15.2 The Central Dogma of Molecular Biology -Crick’s idea was that different combinations of bases could specify the 20 amino acids. DNAas the Intermediary between Genes and Proteins -Jacob and Monod predicted that mRNA carry information from DNAto the site of protein synthesis. -RNApolymerase synthesizes RNAmolecules according to the information provided by the sequence of bases in a particular stretch of DNA. Does not require primer. Question: How is information transferred from DNAto RNA? Hypothesis: RNAis synthesized by complementary base pairing with DNA. Null hypothesis: RNAis not synthesized by complementary base pairing with DNA. Experimental setup: Reaction mix using: 1. Ribonucleotides (A,U,C,G) 2. RNApolymerase 3. DNAstrand with thymine as the only base. Prediction:An RNAstrand will be produced containing only adenine. Prediction of null hypothesis: No RNAstrand will be produced, or an RNAstrand with a random assortment of bases will be produced. Results: RNAstrand with adenine as the only base. Conclusion: Information is transferred from DNAto RNAvia complementary base-pairing. The Central Dogma Francis Crick: DNAcodes for RNAwhich codes for protein. DNA RNA protein DNA is the hereditary material. Genes consist of specific stretches of DNA that code for products used in the cell. The sequen
More Less

Related notes for BIO152H5

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.