BIO152H5 Chapter Notes - Chapter 15: Null Hypothesis, Central Dogma Of Molecular Biology, Genetic Screen
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Knockout mutants/null mutants/loss-of-function mutants: alleles that do not function at all. One-gene, one-enzyme hypothesis: genes contain the information needed to make proteins, many of which function as enzymes. Genetic screen: technique for picking certain types of mutants out of many thousands of randomly generated mutants. *figure 15. 2 experimental support for the one-gene, one-enzyme hypothesis. Crick"s idea was that different combinations of bases could specify the 20 amino acids. Dna as the intermediary between genes and proteins. Jacob and monod predicted that mrna carry information from dna to the site of protein synthesis. Rna polymerase synthesizes rna molecules according to the information provided by the sequence of bases in a particular stretch of dna. Hypothesis: rna is synthesized by complementary base pairing with dna. Null hypothesis: rna is not synthesized by complementary base pairing with dna. Dna strand with thymine as the only base. Prediction: an rna strand will be produced containing only adenine.
