Textbook Notes (363,126)
Canada (158,212)
Biology (653)
BIO153H5 (147)
Chapter Final

BIO153H5 exam review -all the chapter readings-

178 Pages
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University of Toronto Mississauga
Christoph Richter

MicroscopyJanuary10121004 AMProEukaryotic cells range0001 005 mm in diameter only very largest cells 01mmOcular eye piece most have magnification of 10x1 ocularmonocular 1 eye piece2 ocularbinocular 2 eye piecesDont touch ocular lenses with fingersSome oculars have pointer to determine size of specimenMay have trinocular lens for photographyteaching purposesNosepiece Holds objectives and revolves revolve nosepiece to change objectives Objectiveslenses attached to nosepiece DO NOT TOUCH OBJECTIVE LENSES have diff magnification stamped on themScanning Objective Shortest objective view LARGER specimen 4x magnificationLow Power Objectivefor coarsepreliminary focusing 10x magnificationHigh Power Objective For finalfine focusing 40 x magnification of objectOil Immersion objective not on microscope magnification of 100x Oil optical properties used forbetter viewing of specimen after use oil immersion objective must be wiped and cleaned to prevent build upcontamination of other objectivesStagePlatform for slide with slide holder and hole in centre for light to pass throughCoaxial stage control movesfor better viewing of specimen but cant allow objectives to touch stageslideLight SourceSwitchIntensity controlillumination source for microscope switch turns light onoff intensity control depicts how much light is illuminatedIris DiaphragmControls entry of light into microscope mechanical levelrotating diskCondenserlens system beneath stage focuses light on specimenCoarse Adjustment knobuses scanning power only to locate specimenFine Adjustment knobfocus specimen at highlow powersDraw Tube connects ocular to body tubeBody nosepiece on one end and includes draw tubeArm Basesupportive portion of microscope on lab table Lab 1 Notes Page 1 Basesupportive portion of microscope on lab tableUsing A Compound MicroscopeDo not dragmicroscope across table damages opticsmechanisms of the instrumentUse LENS PAPER to clean ocularobjective s Start with scanning objective 4x NEVER START WITH HIGH POWER OBJECTIVEMake sure electric cord is not frayeddamaged and plug in so that it wont get in the waytrip peopledamage microscopeUse slide holder to keep slide in placeAdjust distance between oculars to match distance between eyes and focus oculars for precision using DIOPTER RINGSlocated on each ocularUse coarse adjustment knob to focus specimen ONLY USED TO VIEW SPECIMEN UNDER SCANNING AND LOW POWER DO NOT REST HAND ON STAGEMicroscopes are PARCENTRIC and PARFOCAL if object is centeredsharply focused in one objective it will be the same when another objective is used requires only minimal adjustment USE FINE ADJUSTEMENT KNOB to focus specimen Use IRIS DIAPHRAGM and CONDENSER to centeradjust specimenLab 1 Notes Page 2 Chapter 27January1612907 PM Textbook Page 3
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