BIOA02H3 Chapter Notes - Chapter 1: Agarose Gel Electrophoresis, Sybr Green I, Sucrose
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BIOA02H3 Full Course Notes
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Procedure: put on gloves and grind flies with pestle for about 30 seconds, basic dna extraction: Add 125 ul of sb+ into the tube. Rinse the debris into of the pestle into the tube containing homogenized flies with another 125 ul of sb + Dna) to a new 1. 5 ml tube: add 1/11 volume (18ul ) of 5m nacl to the. 1. 5 ml tube: add 2 volume of ice-cold 95% ethanol (400 ul). Invert test tube and leave it on ice for 5 min: centrifuge the tube for 5 min at 14000 rpm to pellet the precipitated dna. In animal cell: it will break the exoskeleton. In plant cell, it will break down cell wall. Squishing buffer: mixture of weak acid and it"s conjugate base, that minimize the fluctuations in ph when small quantity of acid or base are added.
