BIOA01H3 Chapter Notes - Chapter 12: Phosphorylation, Ribosome-Binding Site, Start Codon

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jadedolphin901

17 Feb 2012

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Bioa01fall2010 chapter 12: from dna to protein: genotype to phenotype. 12. 1 what is the evidence that genes code for proteins. 12. 2 how information flows from genes to proteins. Rna viruses copy their rna and make polypeptides from it. make dna copy of their rna genome and use it to make more rna which is then used as a template for the polypeptides. Rna polymerase recognizes certain bases within the dna douvle helix and binds to them. within each gene only one of the two strands of dna is transcribed -- template strand. Pincers close keeping the dna in double stranded form called a closed complex. Small stretch of dna is denatured forming an open complex. The open complex makes the unpaired bases within dna available to pair with the ribonucleotides and rna synthesis begins. genetic code relates dna to mrna and mrna to amino acids that make up proteins.

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Biochemistry

Which of the following pentapeptides is expected to be the BESTdissolved in water?

Select one:

a. NH2 - ser - pro- gly - ala- cys - asn - COOH

b. NH2 - tyr - thr - gly - ser - gln - asp - COOH

c. NH2 - ser - ala - gly - phe - gln - asp - COOH

d. NH2 - gly - leu - phe - val - ser - gly - COOH

A protein has a molecular mass of 200 kDa as determined by gelfiltration (=native molecular weight). When separated by SDS-PAGE(without a reducing agent present) only one polypeptide appearedwith a mass corresponding to 100 kDa. When determined by SDS-PAGEin combination with a reducing agent (DTT) exactly two polypeptidesappeared of sizes 40 and 60 kDa. What is the correct structure ofthe native protein?

Select one:

a. The native protein is made of one 40 kDA and one 60 kDApolypeptides

b. The native protein is made of two 100 kDa polypeptides

c. The native protein is made of two 40 kDA and two 60 kDApolypeptides

d. The native protein is made of four polypeptides - two 100 kDapolypeptides, one 40 kDA and one 60 kDA polypeptides

e. None of the answers is correct.

ceriserhinoceros720

Describe how information is stored and utilized within a cell and how this information codes for a phenotype. Your description should make use of all of the following terms: DNA, genotype, gene regulation, transcription, mRNA, translation, codon, amino acid sequence (protein), phenotype.

BIOA01H3 Chapter Notes - Chapter 12: Phosphorylation, Ribosome-Binding Site, Start Codon

BIOA01H3 Full Course Notes

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