BIOB10H3 Chapter : chp18 notes.docx
Document Summary
Microscopes are instruments that produce an enlarged image of an object. The phase-contrast microscope makes highly transparent objects more visible. The uorescence microscope allows viewers to observe the location of certain compounds (called. Fluorochromes absorb invisible, ultraviolet radiation and release a portion of the energy in the longer, visible wavelengths, a phenomenon called uorescence. In the late 1950s, marvin minsky of the massachusetts institute of technology invented a revolutionary. New instrument called a laser scanning confocal microscope that produces an image of a thin plane situated within a much thicker specimen. Storm (stochastic optical reconstruction microscopy), which allows investigators to localize a single. Uorescent molecule within a resolution of less than 20 nm. In this technique, the specimen is illuminated with light of different wavelengths, which has the effect of switching the uorescence activity of the labeled molecules on and off.