BIOB12H3 Chapter Notes -Operon, Wild Type, Organelle
Document Summary
Analysis of protein expression in wild type and lactose operon mutant strains using sds-page and xgal/iptg. Week 9a: induction of e. coli cells with iptg and protein sample preparation. Week 9b: separation of e. coli proteins on sds-page and plating cells on xgal- Week10a: analysis of protein expression data with ta and analysis of growth on. Week 10b: presentation of group data to the class using powerpoint. Interpretation of the in-vivo -galactosidase assay using the gratuitous inducer iptg and the substrate x-gal. There are several different biochemical techniques that can be used to purify. In order to study the characteristics or specific proteins in a cell (if unicellular) or. Background and characterize the macromolecules of a cell. For the purposes of this exercise we will concentration on proteins. In addition, you may wish to do subcellular fractionation of eukaryotic cells to allow isolation of particular organelles, such as the nucleus or mitochondria, to assay proteins unique to a particular organelle.
