BIOB12H3 Chapter Notes -Methyl Violet, Microscope Slide, Oil Immersion
Document Summary
Use an ocular micrometer and stage micrometer to determine the size of bacteria and s. cerevisiae. Use of a counting chamber to determine the concentration of an overnight culture of s. cerevisiae cells. How to use a light microscope, major skill for cell biology today. How to use an ocular and stage micrometer. Compare relative size of bacteria (cocci, rod, spiral) to the eukaryote s. cerevisiae. Prepared s. cerevisiae slide and three types of bacteria. Use of the ocular and stage micrometer to size three types of bacteria (rod, cocci and spiral) and s. cerevisiae. The ocular micrometer is a ruler installed in the microscope eyepiece. The ruler has uniform spacing but of unspecified graduations. The ocular must therefore be calibrated using a stage micrometer slide. The stage micrometer is a slide containing a ruler with 10 m spacing in its smallest divisions.
