Laboratory Exercise - Week 11.pdf

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Department
Biological Sciences
Course
BIOB12H3
Professor
Daman Bawa
Semester
Summer

Description
Lab week 11Microscopy moduleOutline11A Use an ocular micrometer and stage micrometer to determine the size of bacteria and S cerevisiaeUse of a counting chamber to determine the concentration of an overnight culture of S cerevisiae cells ObjectivesHow to use a light microscope major skill for cell biology todayHow to use an ocular and stage micrometer Compare relative size of bacteria cocci rod spiral to the eukaryote ScerevisiaeHow to use a counting chamberUnderstand concepts of magnification and resolution Supplies Microscope with ocular micrometer Stage micrometer slide One yeast culture per lab Sterile water Premade Methyl Violet stain Petroff Hausser counting chamber Sterile Microfuge tube Pasteur pipets Hand counter Oil immersion Prepared S cerevisiae slide and three types of bacteria 1
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