NROB60H3 Chapter 6: Neurotransmitter Systems
Document Summary
Molecule must be synthesized and stored in presynaptic neuron. Must be released by presynaptic axon terminal upon stimulation. When experimentally applied must produce a response in postsynaptic cell that mimics response produced by release of neurotransmitter from presynaptic neuron. Used to anatomically localize particular molecules to particular cells. Neurotransmitter is purified and injected into bloodstream where it stimulates immune response that generates specific antibodies. Can then recover antibodies and tag them then use the tagged antibodies to see which cells contain the transmitter candidate. Can be used for any molecule for which a specific antibody can be generated. Can also be used to localize synthesizing enzymes. Create a probe (complementary strand of mrna) chemically label (by making it radioactive)it and allow it to stick to mrna for a particular peptide. Hybridization: the process by which the probe binds to the mrna. Then wash away probe and search for neurons containing label.
