PSYB64H3 Chapter Notes - Chapter 1: Positron Emission Tomography, Horseradish Peroxidase, Fluorescence Microscope
Document Summary
Histology: the study of cells and tissues at the microscopic level. Human eye can see objects that"s at least 0. 2mm in size. A light microscope cannot go beyond 1000x magnification. Electron and fluorescent microscopy can resolve images between 1-100 nanometers (1 million times more powerful than the naked eye. Fluorescent microscopy works by labeling a specimen with a particular fluorophore (chemical that emits light when excited by light: disadvantage: you have to work quickly. Once the tissue is fixed, it"s sliced by a microtome. Optical imaging allows us to see through the brain by removing the fat, which will reduce the scattered light allowing the brain to be transparent. It can provide information about the level of activity for a certain neurochemical in the brain. This also helps to document the precise timing and localization of brain activity. Magnetoencephalography (meg: record brain"s magnetic activity, the advantage is that it there"s no interference between the scalp and the skull like the.
