Chapter all: Visualizing the Cell

244 views14 pages

grayraven524

1 Dec 2016

School

Department

Course

Professor

For unlimited access to Textbook Notes, a Class+ subscription is required.

shdhhfhshhpla3806 and 40102 others unlocked

BIO120H1 Full Course Notes

Verified Note

36 documents

Document Summary

Visualizing the cell, light microscope is a type of instrument that is being used for magnifying small objects that are not visible to human eyes, by using the simple light with magnifying lenses. Light microscope is fundamental instrument for cell biology and used to see the cells of organisms by magnifying them. Single cell organisms can be identified under light microscope (figure9. 1). Light microscope has ability to visualize the bacterial cells that are almost 10-20 m, that are the larger ones. Figure9. 1: light microscopy of bacteria showing single cell organisms: electron microscope is an instrument to see very tiny particles that are even difficult to see via focal or light microscope. Electron microscope has greater resolution power than light microscope and provides us with very fine view of objects under study. Figure 9. 2: electrone microscope: units of length are used to describe the measurement of the object. Here in microscopy measurement units of length are derived.

Related Questions

26. Which of the following is the best microscope to use to visualize a cross section of skin
stained so that the cells are purple and the extracellular matrix is pink:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
27. Which of the following is the best microscope to use in order to visualize cell to cell
junctions at high magnification:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
28. A student is studying the tensile strength of a new metallic alloy. He wants to visualize
the fracture surfaces following tensile testing. Which is the best microscope for this
application:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
29. A graduate student is studying the degradation properties of a new polymer that she
developed. She adds a fluorescent molecule to the polymer backbone and makes a thin
layer of the polymer on a glass slide. In order to visualize the polymer and measure its
degradation/disappearance over time, which is the best microscope to use:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
30. Endothelial cells were exposed to experimental conditions and are imaged intact under a
microscope following antibody labeling of NF-ÎºB, a protein located inside endothelial
cells. Select the following statement that best describes the approach that should be
used to image the endothelial cells in this experiment.
a. Cells are imaged under a transmission electron microscope.
b. Cells are imaged using phase-contrast in the light microscope.
c. Cells are imaged under a fluorescence microscope.
d. Cells are imaged under a scanning electron microscope.

QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

	a.	an isotope
	b.	anisotropic
	c.	isotropic
	d.	divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

	a.	comparator
	b.	comparison bridge
	c.	spectroscope
	d.	stage

QUESTION 6

A stereo microscope can best be described as _____________________.

	a.	Two compound microscopes aligned so that they each see a slightly different part of an object.
	b.	Two compound microscopes aligned with a comparison bridge.
	c.	A compound microscope with two separate stages and one ocular.
	d.	A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

	a.	The ability of the lenses to separate two tiny details that are close together.
	b.	The total magnification power of the microscope.
	c.	The empty magnification power of the microscope.
	d.	The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90Â° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

limecheetah620

QUESTION 1

The ocular of a compound microscope has a magnification of 10x and the objective has a magnification of 10x. The total magnification of the microscope is ___---

	a.	10x
	b.	20x
	c.	100x
	d.	1000x

QUESTION 2

The part of the microscope that focuses the light on an object is the ___________.

	a.	Iris Diaphragm
	b.	Coarse Focus
	c.	Condenser
	d.	Body Tube

QUESTION 3

The polarizing filter in a PLM that is located above the objective is called the _________________.

	a.	Polarizer
	b.	Analyzer
	c.	Abbe condenser
	d.	Iris

QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

	a.	an isotope
	b.	anisotropic
	c.	isotropic
	d.	divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

	a.	comparator
	b.	comparison bridge
	c.	spectroscope
	d.	stage

1 points

QUESTION 6

A stereo microscope can best be described as _____________________.

	a.	Two compound microscopes aligned so that they each see a slightly different part of an object.
	b.	Two compound microscopes aligned with a comparison bridge.
	c.	A compound microscope with two separate stages and one ocular.
	d.	A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

	a.	The ability of the lenses to separate two tiny details that are close together.
	b.	The total magnification power of the microscope.
	c.	The empty magnification power of the microscope.
	d.	The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90Â° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

maroonmuskrat824

Chapter all: Visualizing the Cell

BIO120H1 Full Course Notes

Document Summary

Get access

Related textbook solutions

Molecular Cell Biology

Biology: Science for Life with Physiology

Concepts of Biology

Biology

Essentials of Biology

Related Documents

BIO120H1 Chapter Notes - Chapter 6: Tight Junction, Macrophage, Vacuole

BIO120H1 Lecture Notes - Lecture 17: Trp Operon, Lac Repressor, Lac Operon

BIO120H1 Lecture Notes - Growth Medium, Glucocorticoid, Lac Repressor

Related Questions