BCH311 Andreopoulos.pdf

19 Pages
374 Views
Unlock Document

Department
Biochemistry
Course
BCH311H1
Professor
Stavroula Andreopoulos
Semester
Winter

Description
BCH311 AndreopoulosTranscriptionFunctionInitiation sites and cisacting elements Cis same strand of DNAUnwind dsDNA5 3 Can transcribe without pausing or primer51 in 10error no proofreadingDNAP 800 ntsRNAP 50 ntsFormation of phosphodiester bondsNucleophilic attack by 3 oxygen to Alpha phosphateE coli RNAP400kDaHoloenzyme 270Core enzyme 2has catalytic activity70decreases DNA binding affinityReleased after 10nthelps find the promoterSubunitGeneNumberMass kDaRhoA237RhoB1151RhoC115570RhoD1702 metal ions stabilize with 3 Asp residuesIncoming ribosenucleoside brings in 2nd MgPromoter sequences recognized by RNAPRNA Translation BCH311 Page 1 Initiation Prokaryote promoter sites10 10nt upstream conserved TATAAT Pribnow Box35 another promoter region40nt for core promotersubunit helix in promoter recognitionStrong promoters frequent transcription more substitutions less transcription1720nt of conserved sequenceUpstream elements4060 nts up from start siteCterm subunit binds BCH311 Page 2
More Less

Related notes for BCH311H1

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit