CSB201H1 Chapter : sdfsa.docx
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Hypothesis I: The same histidine synthesis gene is defective in both strains B and C.
Hypothesis II: Different histidine synthesis genes are defective in strains B and C.
Results:
Strain | Growth on SD + HIS | Growth on SD |
B | - | - |
C | + | - |
B x C | + | + |
In Question Set 4, two hypotheses were discussed regarding the HIS mutations in strains B and C. Do your SD plate and SD+HIS plate results support Hypothesis I or Hypothesis II from Question Set 4?
3. A. ) Keeping in mind your answer to question #2 above, what is the predicted genotype of the diploid arising from the BxC mating?
B. ) Can you explain your SD plate and SD+HIS plate results in regards to the URA and LEU genotype of the new diploid that you have made?
Strain | SD+HIS 24 and 48hrs | SD+HIS+URA | SD+HIS+LEU | SD+HIS+URA+LEU |
A | +/+ | +/+ | +/+ | +/+ |
B | +/+ | +/+ | -/- | +/+ |
C | +/+ | -/- | +/+ | +/+ |
1) How many different auxotrophic phenotypes are represented in your collection of three yeast strains?
2)Suppose you had a fourth yeast strain that was haploid and unable to synthesize the amino acid, arginine. If you replica plated the strain onto the same kinds of plates that you used in this experiment, on which plates would this strain be able to grow? Explain your answer.