IMM250H1 Chapter Notes - Chapter 3: Collagen, Tlr5, Defensin
Document Summary
Gram-stain: gram positive (violet) more peptidoglycan, and lipoteichoic acid, gram negative (pink) peptidoglycan is between outer/inner layers, much thinner thus doesn"t retain violet colour. Detect bacteria resistant to typical staining procedures, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid: acid-fast (red, non-acid-fact (blue) , no mycolic layer. Non self, not found in host cells. From self-molecules that are dying or environmental substances. Atp, k+, uric acid, stress (hsps), alum, silica, asbestos. Once they bind with pamps = phagocytosis or complement activation. Collectins: lectin binding domain & collagen domain, make multimeric tulip structures. Cell-signaling prrs made by innate leukocytes and other cells. Cytosolic or membrane bound: toll-like receptors (tlr, nod-like receptors (nlr) Toll-lacking flies infect with disease, they all died. Conc: flies use toll to fight fungi and it is needed in embryonic development & adult immunity. Medzhitov & janeway found human toll, tlrs are everywhere.
