CHEM 437 Chapter Notes - Chapter 1: Förster Resonance Energy Transfer, Surface Plasmon, Scintillation Counter
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Introduction: pi: isoelectric point = where molecule has not neutral charge. Section 2: assays and the meanings of their outputs: Kassociation (kassoc) = inverse of the dissociation constant ka= [rl]/[r]*[l] The smaller the ki, the greater the binding a nity and the smaller amount of medication needed in order to inhibit the activity of that enzyme. Ic50=the ic50 is the concentration of an inhibitor where the response (or binding) is reduced by half. Ec50= the ec50 is the concentration of a drug that gives half-maximal response. Ld50 = lethal dosage for 50% of animals die. In any case the change in [ti] up titration is characteristic of the keq. Power required to keep sample cell at same temperature a reference cell. If ligand binding is exothermic then power is decreased upon heat evolution. G and s are known from: g= h-t s. A strong binding interaction will give a squared titration curve. Indicates a soon as you titrate molecule binds.
