MICR 303 Chapter Notes - Chapter chapter 3: Elution, Ammonium Sulfate, Titration
13 Jan 2018
School
Department
Course
Professor
Document Summary
Ch. 3 + section 3 notes (condensed and combined :) ) Puri cation of proteins is important because when we isolate a protein we can start to analyze how it functions and what mechanisms it uses. inherent properties: Size, hydrophobicity, polarity (charge), pi: enzymatic propertied. We can take advantage of the enzymatic mechanisms (they may be unique: engineered properties. Can arise through dif. cloning technique or introduction of different amino tags. Prediction -> the rst step for any puri cation. Is we know the amino acid composition and # of amino acids, the mass, predicted pi (theoretical not measure) Using a280 (tryp and tyr), can predict the amount of uv each molecule will absorbs, will tell you this much protein absorbs this much light. @ which protein precipitates varies between proteins hence salting out can be used to fractionate proteins. Useful for concentrating dilute solutions of proteins (includuding active fractions obtained from other puri cation steps.
