Biochemistry 2280A Chapter Notes - Chapter 10: Restriction Enzyme, Polyacrylamide Gel Electrophoresis, Recombinant Dna
24 Dec 2016
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Recombinant dna technology: have allowed us to understand the organization and evolutionary history of complex eukaryotic organisms: can be used to detect mutations. Oldest case: of manipulating dna for humans is breeding plants and animals. Restriction nucleases: cut double stranded dna at particular sequences. Restriction nucleases cut dna molecules at specific sites: Some enzymes cut straight across the double helix blunted ends. Other enzymes cut staggered = sticky ends with overhangs: may allow rejoining this way. Bands of dna in a gel can be visualized using. Hybridization provides a sensitive way to detect specific. Southern blotting: the mixture of double stranded dna fragments generated by restriction nuclease of. Northern blotting: rna through gel instead of dna, don"t need to denature since already single stranded, still single stranded dna probe. The production of many identical copies of a dna sequence. Dna cloning begins with genome fragmentation and production of recombinant dnas:
