Biochemistry 2280A Chapter Notes - Chapter 10: Nucleic Acid Hybridization, Sanger Sequencing, Dna Polymerase
25 Dec 2016
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Recombinant dna technology: modification of dna through inserting any sequence into a desired genome. Selective breeding: old fashioned way of manipulating dna. Restriction endonucleases: enzymes that cut dsdna at particular sequences. Can be used to produce set of specific dna fragments from any genome. Restriction nucleases cut dna molecules at specific sites. Restriction nucleases: enzymes function to restrict transfer of dna between strains of bacteria. Do not cut self dna as it is chemically modified at the specific sequences these enzymes cut. Size of resulting fragment: depends on target sequence of restriction nuclease. Longer target sequence: more probable to get larger fragments. Gel electrophoresis separates dna fragments of different sizes. Gel electrophoresis: separates fragments on the basis of length. Isolation: small section of gel that contains dna is excised and dna extracted. Bands of dna in a gel can be visualized using fluorescent dyes or radioisotopes. Dna bands are not visible on the gel alone.
