Purification of osprase involves the following steps: homogenization, ammonium sulfate fractionation, gel filtration chromatography, ion exchange chromatography, and affinity chromatography.a) During the ammonium sulfate fractionation step you lost proteins based on their differential solubility in increasing salt concentrations, a procedure called salting out. Describe this procedure, as well as how you removed the salt afterwards.b) In the gel filtration chromatography step you collected fractions based on the presence of protein as determined by UV absorbance; five peaks of absorbance were observed, with osprase activity found in fraction 2. Explain why UV absorbance is a good way to locate proteins in solution. Sketch a plot of absorbance vs. total volume eluted. What does the fact that osprase eluted from the column in the second peak tell you?c) In a short paragraph, describe the principle of either ion exchange or affinity chromatography.