Describe how to construct a genomic library. When genome is cut by restriction nuclease, millions of diff dna fragments are generated. All the dna fragments are ligated into plasmid vectors. Each e. coli cell takes up one plasmid. Collection of cloned dna fragments in this bacterial culture is called a genomic library reps the entire genome of that organism: outline the strategy for sequencing a small genome. Shotgun method; breaking dna molecule into fragments, determining the sequence of each one, using computer to search for overlaps and build the master sequence. Aligning the independent sequences into a continuous sequence: describe the approaches used to fill in any gaps in the genome sequence. Sequence gaps: clones whose 2 end-sequences were located in diff contigs. Additional sequencing of its insert would close gap using primers internal to fragment. Made possible by originally sequencing both ends of the contigs: physical gaps: stretches of sequence not present in clone library.