Biology 2581B Chapter Notes - Chapter 15 + Restriction Mapping: Recombinant Dna, Plasmid, Sticky And Blunt Ends
Document Summary
Restriction fragments: fragments generated by the digestion by restriction enzymes. Restriction enzymes originate from bacteria (who can shield their dna from digestion with methyl groups: palindromic sequences are recognized and cut always in the same way. Modification: host modification of a virus to make them more effective in growing in subsequent generations. Can be done one a fragment of dna but cutting with different restriction enzymes. Molecular cloning: take fragments of dna of interest and break by restriction enzymes. Insert the fragments into vectors which ensure transport, replication and purification of the dna of interest: vector: a specialized dna sequence that can enter a living cell, signal presence to host cell and provide means of replication. Plasmids are most frequently used: recombinant dna molecule: both the vector and the inserted fragment. This is done by sticky ends match up with the insert: ligase must be used to seal the gap.