Biology 2581B Chapter Notes - Chapter 8,9,17: Transposable Element, Transpose, Reverse Transcriptase
4 Jun 2017
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Chapter 8 p 257 chapter 9 p 294-297 chapter 17 p 557-558. Transposition: the movement of small segments of dna entities (transposable elements) from one position in the genome to another. Transposons: any segment of dna that evolves the ability to move from place to place within a genome. Mammals carry lines or sines: line: long interspersed elements, sine: short interspersed elements. Transpose via reverse transcription: of an rna intermediate: enzyme copies lines and sines, retrotransposons undergo replicative transposition: make a copy that becomes inserted into a new site while the initial site is still there, copy and paste . Undergo conservative transposition: removed from original site and transferred into new site. Embryonic stem cell from inner cell mass of blastocyst from mice are treated with cloned exon disrupted with transposon (we are calling the transposon neo) Embryonic cells are pluripotent and useful but need to destroy a blastocyst to obtain. Adult stem cells are ok but only multipotent.
