Biology 2581B Chapter Notes - Chapter 8 (Kolhami): Replica Plating, Bactericide, Purine
4 Jun 2017
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Forward mutations: change from a wild type allele to a different one. Reverse mutations: novel mutant allele back to wild type. Different types of mutations: substitution, transition: purine to purine, transversions: purine to pyrimidine, vice versa, insertions, deletions, inversions, flipping a gene, reciprocal translocations, parts of non homologous chromosomes change places. To find whether mutations are random or in response to something. Was found that bacterial resistance arises from mutations that exist before exposure to bacteriophage. Bactericide acting as selecting agent for replica plating method: Showed that gaining the mutation was stable, same spot on respective dishes: used velvet plate to make replicate cell plates, same areas of each plate had bactericide resistant bacteria, indicate not as a result to environmental change. Dna polymerase has a 3" to 5" exonuclease that repairs so that only 106 bases there is a mistake. Unequal crossing over one chromosome gets deleted and other gets insertion if the lengths of chromosomes are different.
