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Physiology (97)
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Type of Experiment.docx

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Department
Physiology
Course
Physiology 3140A
Professor
Donglin Bai
Semester
Fall

Description
Type of Experiment Technique Advantages Disadvantages Sharp Electrode Recording/Current-Clamp • High resistance electrode • Simple direct • Limited control of (high noise) measurements of voltage membrane • Membrane seals around with respect to time potential (hard to electrode • Emulate in vivo inject current) conditions • High resistance • Adjust amplifier to 0 in bath (small tip) electrode = high noise – unable to distinguish single channel activities Voltage-Clamp Recording • 2 electrodes – one injecting • Voltage is under control – • Technically current & the other recording important because many demanding cell membrane potential channels are voltage • Voltage clamp amplifier dependent compares membrane • Quantitative measure of potential to desired current or conductance (I (command) potential = V x G therefore I α G) • When membrane potential is • Current imposed by different from command channels can be
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