BIOL 1000 Chapter Notes - Chapter 15: Agarose, Restriction Enzyme, Electrophoresis

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Biotechnology: any technique applied to biological systems or living organisms to make or modify products or processes for a specific purpose. Dna technology: techniques used to isolate, purify, analyze, manipulate dna to serve a specific purpose. Genetic engineering: altering genes of genomes for practical purposes. Example: e. coli - e. coli and molecular biology = biotechnology: beneficial (in intestines) - rod shaped multi flagellum organism, can isolate these on a median and grow these bacteria. Dna cloning: creating multiple identical copies of a piece of dna (gene). Recombinant dna: joining dna from two different sources together. Common methodology for cloning dna: put dna in bacteria and then grow lots of bacteria. Recall: plasmid contains nonessential genes: put ge(cid:374)e of i(cid:374)terest i(cid:374)to a plas(cid:373)id (vector)

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