ESPM 108B Chapter Notes - Chapter 5: Pyrosequencing, Transposable Element, Chromatography
23 Dec 2018
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Separated into 2 strands, copied using chemically altered bases. All bases cause copying process to stop each time 1 particular base is added to chain. Carried out for all 4 bases, frags put together by size like a jigsaw. 600-1k bp max, beginning and end poor quality. Today: 24 hr to seq human genome. Attributes of markers for coarse-scale studies : Slower mutation rate - variable due to deeper evolutionary events. Mutation that conforms to model - in nite alleles (iam)/in nite sites (ism) assumes that dna seq are long, so mutation assumed to be at random and chance of mutation at same location is small 0. Markers that allow development of gene genealogies. Have to break down into smaller pieces and put together sequence reads. De novo genotyping : if there"s no ref genome, more tedious alignment, closest relative may be used as a partial ref genome, aimed at providing example of major taxon groups.
