8.4: How do Enzymes Work?
Enzymes can orient substrates: Need to proper orientation to interact.
Enzymes can induce strain in the substrate: stretching the bonds between monomers, to make
bonds unstable and more reactive to the substrate
Enzymes can temporarily add chemical groups to substrates: side chains can make substrates
more chemically reactive
Acid-Base Catalysis: acidic or basic side chains of amino acids in active site transfer H+
to or from the substrate to destabilize covalent bond
Covalent Catalysis: functional group forms a temporary covalent bond
Metal ion catalysis: metal ions gain or lose electrons without detaching from enzymes
Molecular structure determines enzyme function
Active site is specific to substrate. Meaning, that depends on tertiary structure of the
enzyme: hydrogen bonds, attraction/repulsion of electric-c