BIOL 22000 Chapter Notes - Chapter 8: Membrane Protein, Action Potential, Agonist

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Lecture 8
How do cells pick up information from the environment and respond appropriately?
o Fundamental problem for all life
o Cells need to translate an external signal into an internal change
o Cells can electrical or chemical to relay messages, sometimes over long distances
o Similar messages used in all forms of multicellular life
Within A Single Cell
Between Cells
Electrical Communication
Action Potential, Graded
Potential
Electrical Synaptic
Transmission
Chemical Communication
Cell Signaling
Chemical Synaptic
Transmission, Endocrine
Signaling
Basics of Cellular Signaling
o Cellular processes can change in response to both chemical and electrical
signaling
Example of electrical signaling is graded potential can change
permeability of the cell to ions
o Chemical signaling requires receptor
A protein act as a receptor to bind to the initial chemical signal
o The chemical that interacts with the receptor is called a ligand
Sometime ligand is called first messenger
o Both endogenous and exogenous ligands can activate receptors
Endogenous: a molecule that is produced within your own body
Exogenous: a molecule that comes from outside the body and can bind to
your receptor
Sometime more then one of these can activate the receptor
o The specific cellular responses to the ligand are determined by the
receptor, NOT ligand
Sometime the protein that relay the message from the receptor to the rest
of the cell can also determine the response
o In most cases, the chemical message is relayed within the cell and
translated into cellular change using positive feedback
The flow chart
o First a receptor protein, either endogenous or exogenous ligand, aka first
messenger, binds to a receptor protein
o The receptor protein is usually found on the cell membrane
o The binding of the ligand to causes conformation for the receptor protein
This cause signaling cascade of changes to other protein and molecules
o The activates signal molecule alters the target protein
Example: protein that is more transcription or translation, ion channel.
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o The first one is simply an ion channel and bypasses all the middle step
A chemical bind to the receptor and causes a chemical change, and the
receptor open and closes
Produced a change right there
o The second one is called G-Protein receptor
A receptor binds to the outside of the protein and cause a conformational
change
The conformation change also causes the conformation for the protein that
is associated with the intracellular side of the receptor, aka G-Protein
G-Protein is like a relay mechanism that activates the other changes in the
cell
o The third one is called Receptor-Enzyme
The receptor itself has enzymatic activity
When the ligand binds to the receptor on the ECF, it activates the enzyme
activity in the ICF
A lot of time the activity is kinase activity, that means when activated the
intracellular part can add phosphate group to different molecule/receptor
The present/removal of the phosphate is associated with active and
inactive
o The forth one is Integrin Receptor
The ligand attached to the receptor translate to a change in cytoskeleton, to
allow the cell to move and change shape
NOT TALKED ABOUT
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o Receptor Channel: nAChR
The receptor is going be usually bound by an endogenous ligand called
acetylcholine (neural transmitter)
Since is an ion channel, it skips some of the steps on the flow chart, such
as intracellular signal molecules
Nicotinic Acetylcholine receptors is expressed on skeletal muscle cells
Receptor is nonselective cation specific ion channel
Let any cation ion through, such as potassium and sodium
Ligand binding opens channel
Increased membrane permeability to both K+ and Na+
Depolarizing graded potential
Muscle contraction
Most cells have higher permeability to potassium then to sodium
o
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