BIO 226 Chapter Notes - Chapter 7: Asepsis, Disinfectant, Incineration

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Disinfection- removal of pathogens on inanimate objects. Antisepsis- removal of pathogens on living tissue. Degerming- removal of microbes from a limited area. Sanitization- lower microbial counts on eating utensils. When bacterial populations are treated with microbial control agents every minute, the death rate is constant and results in a straight line. Factors that influence the effectiveness of antimicrobial treatments: Alteration of membrane permeability: damage to the lipids or proteins of membrane causes the cellular contents to leak into the surrounding medium which interferes with cellular growth. Damage to proteins: heat or certain chemicals can result in denaturation of the proteins. Damage to nucleic acids: heat, radiation, or chemicals is lethal to the cell which disables cell replication and metabolic activity. Boiling- type of sterilization that kills pathogens, almost all viruses, and fungi and their spores within about 10 min; not always reliable.