BIOL 142 Chapter 19.4: Post-Transcriptional Control

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19.4%Post-Transcriptional%Control%
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!"#$%$&'(#)&*+$*"(',-)"($&",:!any!regulation!that!occurs!after!transcription!
Once!a!gene!is!transcribed,!a!series!of!steps!have!to!occur!before!there's!a!final!product!
Control!mechanisms:!
1. Different!ways!of!splicing!the!same!RNA!primary!transcript!
2. Inhibiting!the!translation!of!most!mRNAs!in!the!cell!
3. Destroying!particular!mRNA!or!altering!the!ability!to!translate!them!
4. Altering!the!activity!of!proteins!after!translation!has!occurred!
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.,$/&('$*0/-1+,*)*(2-"3-!&*4'&5-6&'(#)&*+$#!
Introns!are!spliced!out!in!the!nucleus!as!the!primary!RNA!is!transcribed!
mRNA!that!results!from!splicing!
o Consists!of!sequences!encoded!by!exons!
o Is!protected!by!a!cop!on!the!5'!end!and!a!long!poly(A)!tail!on!the!3'!end!
1+,*)/"#"4/#:!macromolecular!machines!that!cause!splicing!
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.,$/&('$*0/-#+,*)*(2:!in!eukaryotes,!the!splicing!of!primary!RNA!transcripts!from!a!single!gene!in!different!
ways!to!produce!different!mature!mRNAs!and!thus!different!polypeptides!
Many!primary!transcripts!can!be!spliced!in!more!than!one!way!-7*2-#"8&)/-"3-&/28,'$*"(-*(-
/89'&5"$*)-2/(/-/:+&/##*"(!
o During!splicing,!gene!expression!is!regulated!by!differential!processing!of!introns!and!exons!
o Results!in!the!same!primary!RNA!transcript!being!spliced!together!in!different!ways!
;*/,<#-4"&/-$='(-"(/-9*(<-"3-4'$8&/>-+&")/##/<-4?@.!
o Since!these!mRNAs!contain!differences!in!their!sequences,!the!polypeptides!translated!from!
them!will!differ!!
Muscle-cell!protein!tropomyosin!
o The!tropomyosin!gene!is!expressed!in!two!distinct!kinds!of!muscle!cells:!
Skeletal!muscle!responsible!for!movement!
Smooth!muscle!that!lines!the!gut!and!certain!blood!vessels!
o Tropomyosin!contains!14!exons,!but!each!type!of!muscle!cell!has!a!different!subset!of!the!14!
exons!present!in!the!primary!transcript!
Produce!two!different!mRNAs!
o Because!of!',$/&('$*0/-#+,*)*(2,!the-#'4/!tropomyosin!2/(/!in!skeletal!and!smooth!muscle!
cells!is!expressed!differently!to!+&"<8)/-$A"-<*#$*()$-+&"$/*(#!
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Document Summary

Post-transcriptional control: any regulation that occurs after transcription: once a gene is transcribed, a series of steps have to occur before there"s a final product, control mechanisms: Inhibiting the translation of most mrnas in the cell: different ways of splicing the same rna primary transcript, destroying particular mrna or altering the ability to translate them, altering the activity of proteins after translation has occurred. Introns are spliced out in the nucleus as the primary rna is transcribed: mrna that results from splicing , consists of sequences encoded by exons. Is protected by a cop on the 5" end and a long poly(a) tail on the 3" end. Can transcribe the same gene into the same primary rna transcript but splice the primary transcript in different ways. The number of different proteins and mature rnas produced is far larger than the number: alternative splicing is a major mechanism in the control of gene expression in multicellular of genes eukaryotes.

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