MCB 3020 Chapter Notes - Chapter 10: Human Genome Project, Intron, Genomic Library
Document Summary
Types of cloning vectors: plasmids (most commonly used, phages and viruses, cosmids, artificial chromosomes. Plasmids: replicate autonomously and easy to purify, requirements for vectors. Site that allows gene to be cloned to placed into the plasmid. Phage vectors vector: are engineered phage genomes previously genetically modified to include restriction sites, after insertion of foreign dna the recombinant phage genome is packaged into the capsid and used to infect host cells. Cosmids: do not exist in nature, these vectors have been constructed to contain features from both phages and plasmids they have a selectable marker, multiple cloning sites from plasmids, and a cos site from l phage. Phage then introduces recombinant dna into e. coli. Artificial chromosomes: used when large fragments of dna must be cloned, bacterial artificial chromosomes (bacs) Played important role in the human genome project: yeast artificial chromosomes (yacs) May be unstable, for very large dna fragments.
