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Chapter 16

MCB 3020L Chapter Notes - Chapter 16: Reaction Rate, Ideal Gas Law


Department
Microbiology
Course Code
MCB 3020L
Professor
Scheurle Daniela
Chapter
16

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Chapter 16
Chapter 16: Sterilization
Sterilization: the complete elimination of vegetative bacterial cells
o Irridation, heat, high pressure, filtration, or chemicals
Ionizing radiation (X rays or gamma rays)
o Are both commonly used to sterilize items such as disposable medical tools and
equipment
o As the radiation pass through the microbial molecules, they force electrons out
of their shells, creating ions
These ions quickly combine (mostly with cellular water) and free radicals
are generated
Free radicals can affect cell metabolism and physiology
o Ionizing radiation does NOT have a direct effect on nucleic acids (such as DNA or
RNA)
o Advantages: The items being sterilized remain at normal temperatures and
containers are able to remain completely sealed throughout the sterilization
process due to the penetrating abilities of the ionizing radiation passing through
them
Heat: probably the most common form of sterilization
o Incinerators!
o As the temperature increases, the rate of reaction increases until the
temperature is high enough that the enzymes become denatured and the
reaction rate falls
o At high temperatures, even enzymes of thermophilic bacteria will eventually
become denatured and the bacteria will eventually be killed as their enzymes
become dysfunctional
o MOIST heat (in the form of steam) is much more effective than DRY heat
Steam sterilization- accomplished with the use of an autoclave
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