MCB 2000 Chapter Notes - Chapter 17: Syphilis, Restriction Enzyme, Complement Fixation Test
Document Summary
How to identify the disease: direct testing (microscopic, immunological, genetic methods) Time required: from few minutes : cultivation of microorganisms. Time required: few days to few weeks (mycobacterium tuberculosis: based on symptoms (athlete"s foot) How to identify unknown bacteria: phenotypic methods. Looking for the specific antibody in patient"s blood. Immediate direct examination of specimen: direct microscopic observation. Stained (gram staining, acid fast staining: direct fluorescence antibody test. Cultivation of specimen: a dominant organism has to be isolated into a pure culture, the isolated microorganism is characterized by. Phage typing: identifies which phage affects specific bacterial strain, can distinguish strains within one species, enables tracking the source of the outbreak. Agglutination reactions: particulate antigens (microbial cells) react with antibodies forming visible aggregates. Precipitation reaction: soluble antigens react with antibodies forming large molecular aggregates visible precipitate, there are two techniques: Complement fixation test: components needed for this test. Sensitized sheep red blood cells: conducted in two stages.
