BIOL 2344 Chapter Notes - Chapter 24: Gene Family, Gene Knockout, Protein Microarray
Document Summary
Dna microarray can quantify gene transcription at whole genome level: dna microarray ~ small silica, glass, or plastic slide that is dotted with many different sequences of dna, each representing short sequence within known gene, gene chip. Gene knockout collections allow researchers to study gene function at genomic level: gene knockout ~ in case of diploid species, condition in which both copies of gene have been altered to inactive form. Two-dimensional gel electrophoresis is used to separate a mixture of different proteins: two dimensional (2d) gel electrophoresis ~ technique to separate proteins that involves isoelectric focusing followed by sds- gel electrophoresis. Isoelectric focusing ~ form of gel electrophoresis in which protein migrates to point in the gel where its net charge is zero. Sequence files are analyzed by computer programs: computer programs ~ series of operations that can manipulate and analyze data in desired way, computer data file ~ collection of information stored by computer.
