BIOL 3450 Chapter 4: Chapter 4 – Culturing and Visualizing Cells
Document Summary
4. 1 growing and studying cells in culture: study of cells is greatly facilitated by growing them in culture, so they can be examined by microscopy and subjected to specific treatments under controlled conditions. Transformed cells can grow indefinitely in culture to all tissues during development: cell lines ~ population of cultured cells, of plant or animal origin, that has undergone genetic change allowing cells to grow indefinitely. Growth of cells in two dimensional and three dimensional culture mimics the in vivo. Environment: epithelia ~ sheet like layers of epithelial tissue that cover external and internal surfaces of organs, ex. apical (top), basal (base or bottom), and lateral (side) surfaces. Wide variety of cell biological processes can be studied with cultured cells: cultured cell uses, elucidation of fundamental processes. Inactivated specific genes n genomes and assess how complete loss of specific. Drugs are commonly used in cell biological research. 4. 2 light microscopy: exploring cell structure and visualizing proteins within cells.
