BIOL 111 Chapter Notes - Chapter 20: Dna Ligase, Recombinant Dna, Sticky And Blunt Ends

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23 Dec 2019
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Bacterial restriction enzymes cut dna molecules specific dna sequences called restriction sites: a molecular scissors that only works at certain dna sequences. The cut pieces of dna are restriction fragments. The most useful restriction enzymes cut dna in a staggered way, producing fragments with. Sticky ends can bond with complementary sticky ends of other fragments. Dna ligase seals the bonds between restriction fragments: molecular tape , same enzyme in dna replication. Cloning a eukaryotic gene in a bacterial plasmid. In gene cloning, the original plasmid is called a cloning vector. A cloning vector is a dna molecule that can carry foreign into a host cell and replicate there. A bacterial artificial chromosome (bac) is a large plasmid that has been trimmed down and can carry a large dna insert. Bac"s are another type of vector used in dna library construction.

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